Isolation,Culture and Characterization of Rabbit Adipose-derived Stromal Cells

In order to establish a method for isolation and culture of rabbit adipose-derived stromal cells in vitro and explore their biological characteristics.Inguinal fat pads of rabbit were aseptically separated,followed by digestion with I type collagenase,then cultured in DMEM with 10% fetal bovine serum and assay its growth curve of the third,fifth and tenth generation.To verify the multipotential differentiation of mesenchymal characteristics of ADSCs,the third passage cells were subjected to differentiation in conditions known to induce adipogenic and osteogenic lineages and used for phenotypic analysis by flow cytometry.There were no significant deviation between growth curve of the third,fifth and tenth passage.Cells had a strong capability of proliferation.After cultured in adipogenic differentiation medium,the cells were Oil Red O staining positive.After cultured in osteogenic differentiation medium,the cells were Von kossa staining positive.The cultured ADSCs in this study were positive for CD29,CD90 and negative for CD80 and CD45.ADSCs were successfully isolated and cultured from rabbit adipose tissue.These cells have adipogenic and osteogenic potential.This study showed that the ADSCs could be served as seed cells in tissue engineering.