Role of glucagon-like peptide-1 on proinsulin mRNA levels in diabetic mouse

Objective: To investigate the role of intravenous glucagon-like peptide-1 on blood glucose and on pancreas′s proinsulin mRNA level in diabetic mice.Methods: Alloxan(45?mg/kg) was infused by a tail vein in male Kunming mice to successfully make the diabetes model.Twenty diabetic mice were divided at random into a control group(normal(sodium)) and an experimental group[GLP-1(7-36)NH_2].Tail blood glucose was measured respectively before(infusion) of normal sodium[GLP-1(7-36)NH_2] and at 5 and 10 minutes after infusion.Proinsulin mRNA levels were(assessed) by the semi-quantitative reverse transcriptase polymerase chain reaction(RT-PCR).Results: Blood glucose was not significantly different before intravenation between the control and the experiment groups(P0.05);blood glucose(concentrations) at 5 and 10 minutes after intravenation were significantly decreased in the experiment group compared with the control group((P)0.05).The levels of proinsulin mRNA were significantly higher in the experiment group comparedwith the(control) group((P)0.05).Conclusion: Intravenous injection of GLP-1(7-36)NH_2 can promote proinsulin gene transcripts,(increase) pancreas′s proinsulin mRNA level,stimulate insulin biosynthesis and secretion and quickly decrease blood(glucose.)