2016•Journal of Jilin UniversityRequires access
Effect of astragaloside on oxygen radical metabolism and expression of TGF-β1 mRNA in diabetic nephropathic rats
Huang Xiaodon
Open publisher page 0 citations
Abstract
Objective:To study the effect of astragaloside on the oxygen radical metabolism and(DN)the expression of transforming grow th factorβ1(TGF-β1)mRNA in the rats with STZ-induced diabetic nephropathy(DN),and to clarify its related mechanism.Methods:60 Wistar rats were divided into control group,model group,irbesartan group,low dose(0.75 mg · kg · d~(-1)) of astragaloside group, middle dose(1.50mg·kg·d~(-1))of astragaloside group and high dose(3.00mg·kg·d~(-1))of astragaloside group(n=10).Except control group,the rats in other groups were intraperitoneally injected with 60mg·kg·d~(-1) STZ to induce DN models.8 weeks later,the levels of serum creatinine(Scr),blood urea nitrogen(BUN),fasting blood glucose,and 24 hurinary protein of the rats in various groups were detected.The SOD and GSH-Px activities and MDA levels in kidney tissue of the rats in various groups were measured;the expression levels of CAT and TGF-β1mRNA in kidney tissue were determined by RT-PCT.Results:Compared with model group,the kidney function of the DN rats in middle and high doses of astragaloside groups was improved;the serum Scr levels of the rats in astragloside groups were decreased(P0.05 or P0.01),and the levels of serum BUN and PBG and 24 h urinary protein of the rats in middle and high doses of astragaloside groups were decreased(P0.05 or P0.01).Compared with model group,the MDA levels in kidney tissue of the rats in middle and high doses of astragaloside groups were decreased and the SOD activites were increased(P0.05 or P0.01);the expression levels of TGF-β1mRNA were decreased(P 0.05);the GSH activities in kidney tissue of the rats in various doses of astragaloside groups were increased P0.05 or P0.01);but the expression levels of CAT mRNA had no changes(P0.05).Conclusion:Astragaloside can improve the kidney function of DN rats,its mechanism may be related to the improvement of oxygen radical damage and reduction of TGF-β1mRNA expression.