Effect of XinNaoNing on Apoptosis and Relevant Gene Expression in Cultivated Vascular Smooth Muscle Cell

Objective :To explore the effect of XinNaoNing recipe on vascular smooth muscle cells(VSMC)apoptosis and its molecular mechanisms. Methods :VSMC apoptosis was detected by flow cytometry and DNA agarose electrophoresis.The expression activity of bcl-2 and c-myc genes were determined using Northern blotting. Results: the typicall characteristics of apoptotic VSMC was observed following treatment by the XinNaoNing drug serum.The DNA extracted from VSMC revealed a ladder pattern in electrophoresis.Flow cytometric analysis revealed G 0/G 1 phenomena,the hypoidploid apoptotic cell increased,displayed typical peak of apoptosis,the rate of cell apoptosis and XinNaoNing serum concentration were in a dose-dependent manner.The result of hybridization showed that 30% of XinNaoNing drug serum could inhibit apoptotic gene bcl-2 expression,upregulated the level of apoptogenous gene c-myc mRNA. Conclusion :VSMC apoptosiss could be induced by XinNaoNing recipe and its mechanism might be through affecting the apoptosiss related gene expression activity.