Effects of fibroblast growth factor on rat bone marrow osteoblast in vitro

AIM: To explore the effects of fibr ob last growth factor (FGF) on the induced osteoblast of rat bone marrow-derived m esenchymal stem cells. METHODS: The osteoblasts were induc ed from and expanded in cell culture with dexamedasone, sodium-glycerophosphate and ascorbic acid (Vit C) respectively. The induced cells with different concent rations of FGF (2, 4, 6 and 8 mg·L -1 ) as experimental group and the cells without FGF as control group were compared. The growth curve, activity of alkal ine phosphates (ALP) and osteocalcin (OCN) were observed. RESULTS: After adding FGF with different densities (2, 4, 6 and 8 mg·L -1 ), the growth rate of the cultured cells had no great differentiation ( P0 05). The secretion of ALP decreased from 4 90±0 31 U·L -1 to 2 91±0 21 U·L -1 (P0 05). But the osteocalcin (OCN) secretion was increased from 1 998±0 223 ng·L -1 to 3 701±0 293 ng·L -1 (P0 05). The formation of mineralized node became increased (P0 05). CO NCLUSION: FGF has significant effects on the inducement of Wistar rat bo ne marrow-derived mesenchymal stem cells in vitro. With the conc entration of FGF 2 mg·L -1 , ALP and OCN get to the highest in a dose-depen dant manner. (P0 05).