Effects of midazolam on cell apoptosis induced by hepatic ischemia-reperfusion in rats

Objective To investigate the effects of midazolam on cell apoptosis induced by hepatic ischemia-reperfusion in rats.Methods Fifty-four SD rats were randomly divided into 3 groups(n=18):sham-operated control group(group Sham),ischemia-reperfusion group(group IR)and midazolam group(group M).Establish a rat model of hepatic ischemia-reperfusion.The left hepatic lobes were sampled at the end of reperfusion.Bcl-w and Bax proteix expression in liver tissue was assessed by immuno-histochemical method.The apoptosis index(AI)of hepatic cell were measured by TUNEL method.Results Bcl-2,Bax protein expression and apoptosis index of hepatic cell were statistically different between group Sham and group IR,M(P0.05).Bcl-2 protein expression was significantly higher while Bax protein expression and AI Significantly lower in group M than group IR(P0.05).The result in three time phases of group IR,M reached the peak at 6h after reperfusion.Conclusion Midzazolam can relive the liver cellular apoptosis by regulating Bcl-2 and Bax protein expression.It may protect rat from hepatic inchemia-reperfusion injury.