Effects of Expression of Enhanced Green Fluorencent Protein Gene on C6 Cells
Youwei Wang, Song Yu-guang, HU Huo-zhen, Lin Hong, Xing Yu-zhi, Fen Chen
Abstract
Youwei Wang, Song Yu-guang, HU Huo-zhen, Lin Hong, Xing Yu-zhi, Fen Chen
Abstract
C6 cells were transfected with pEGFP-N1 by lipofectamine Plus.Stable EGFP-expressing cells were isolated with G418.Proliferation,activity and cell cycle of C6-EGFP were evaluated through cell-counting, MTT and Flowcytometry.As C6 cells transfected with pEGFP-N1,the proliferation and activity effects decreased and the cell cycle changed.
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C6 cells were transfected with pEGFP-N1 by lipofectamine Plus.Stable EGFP-expressing cells were isolated with G418.Proliferation,activity and cell cycle of C6-EGFP were evaluated through cell-counting, MTT and Flowcytometry.As C6 cells transfected with pEGFP-N1,the proliferation and activity effects decreased and the cell cycle changed.
Key concepts: Lipofectamine, Transfection, Green fluorescent protein, Cell cycle, Molecular biology, Cell growth, Chemistry, Cell