2006•Journal of Sichuan UniversityRequires access

Effects of Expression of Enhanced Green Fluorencent Protein Gene on C6 Cells

Youwei Wang, Song Yu-guang, HU Huo-zhen, Lin Hong, Xing Yu-zhi, Fen Chen

Open publisher page 0 citations

Abstract

C6 cells were transfected with pEGFP-N1 by lipofectamine Plus.Stable EGFP-expressing cells were isolated with G418.Proliferation,activity and cell cycle of C6-EGFP were evaluated through cell-counting, MTT and Flowcytometry.As C6 cells transfected with pEGFP-N1,the proliferation and activity effects decreased and the cell cycle changed.

About this research paper

What this paper is about

C6 cells were transfected with pEGFP-N1 by lipofectamine Plus.Stable EGFP-expressing cells were isolated with G418.Proliferation,activity and cell cycle of C6-EGFP were evaluated through cell-counting, MTT and Flowcytometry.As C6 cells transfected with pEGFP-N1,the proliferation and activity effects decreased and the cell cycle changed.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

C6 cells were transfected with pEGFP-N1 by lipofectamine Plus.Stable EGFP-expressing cells were isolated with G418.Proliferation,activity and cell cycle of C6-EGFP were evaluated through cell-counting, MTT and Flowcytometry.As C6 cells transfected with pEGFP-N1,the proliferation and activity effects decreased and the cell cycle changed.

Key concepts: Lipofectamine, Transfection, Green fluorescent protein, Cell cycle, Molecular biology, Cell growth, Chemistry, Cell

Related papers

Back to paper searchBrowse research topicsOriginal source
Effects of Expression of Enhanced Green Fluorencent Protein Gene on C6 Cells — Research Paper | ScholarLens