Activating Transcription Factor 5 Invloved in SW1990 Cell Line Apoptosis Induced by Epigallocatechin Gallate

Objective: To study whether activating transcription factor 5(ATF5) involved in human pancreatic cancer cell SW1990 apoptosis induced by epigallocatechin gallate(EGCG).Methods: MTT assay was used to detect viabilities of SW1990 cells after treated with EGCG at different concentrations for different hours respectively.Then SW1990 cells were treated with 100 mg/L EGCG,and morphologic changes were observed by inverted microscope.The cell apoptosis was observed by flow cytometry with SR-VAD-FMK/7-AAD double staining.The changes of ATF5 mRNA expression after treated with 100 mg/L EGCG were monitored by RT-PCR.Results: EGCG effectively inhibited the viability of SW1990 cells in a dose-dependent and time-dependent manner.There was also a significant increasing amount of floating cellular debris in the cultures after treated with EGCG.EGCG promoted apoptosis of SW1990 cells and increased expression of ATF5 mRNA(P0.05).Conclusion: ATF5 expressed in human pancreatic cancer cell SW1990,and the mRNA expression of ATF5 was increased when cells treated with EGCG.Our data indicates that ATF5 involves in the SW1990 cells apoptosis induced by EGCG.