2002•PubMedRequires access

[Detection of the HBV DNA with Sybr green 1 realtime polymerase chain reaction].

Yan Wang, Xiaoyuan Xu, Wei He, Zhihong Liu, Weibo Gong, Qinhuan Wang

Abstract

BACKGROUND: To search a novel sensitive, specific and lower cost method applicable for quantitative analysis of the hepatitis B virus DNA extensively. METHODS: Quantitative analysis of the DNA from 100 sera by real-time PCR with Sybr green 1. The results of Sybr's assay were compared with the results obtained with Taqman's fluorescent quantitative assay. RESULTS: Taqman real-time PCR could help evaluate the level of virus reliably. The results of Sybr's assay were in agreement with the Taqman's assay, but detection rate was lower. CONCLUSIONS: Sybr green 1 real-time PCR appeared to be convenient and cheap, but detection rate was lower.

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Available abstract

Key concepts: TaqMan, SYBR Green I, Real-time polymerase chain reaction, Polymerase chain reaction, Molecular biology, Primer dimer, Virology, Biology