The mechanism of apoptosis in human gastric carcinoma cells BGC-823 induced with recombinant plasmid comprised Hemagglutinin-neuraminidase(HN) gene from newcastle disease virus

Objective To study the mechanism of apoptosis in Human Gastric carcinoma cells BGC-823 Induced with recombinant plasmid comprised Hemagglutinin-neuraminidase(HN) Gene from Newcastle Disease Virus(NDV).Methods The recombinant plasmid pIRVP3IL-18HN was introduced into human gastric carcinoma cells BGC-823 by liposome-mediated transfection.The viability of BGC-823 cells was determined by MTT staining and the morphology of BGC-823 was observed by electron microscope.The alteration of mitochondrial transmembrane potential and ROS level of the cells was detected by flow cytometry(FCM) with Rhodamine 123 and DCFA staining and the activation of caspase-3 was assayed by substrate color reaction.Results After recombinant plasmid was introduced into BGC-823 cells,mortality of cells was increased and viability was decreased significantly respectively.Apoptosis of BGC-823 cells can be observed significantly by electron microscope.Compared with control cells,mitochondrial transmembrane potential was decreased,ROS level of the cells was increased,and caspase-3 was activated.Conclusion A sequencing apoptosis of human gastric carcinoma cells BGC-823 can be induced with recombinant plasmid comprised Hemagglutinin-neuraminidase(HN) Gene from Newcastle Disease Virus,then induce the apoptosis of tumor cells.