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Study on Characteristics of PPO and Anti-browning Inhibitors in Pomegranate

Zhang Li-hua

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Abstract

Spectrophotometer method was applied in this experiment to study the characteristics of polyphenol oxidase(PPO) in pomegranates.This article also analyzed the effects of citric acid,ascorbic acid,sodium sulfite andβ-cyclodextrin on PPO. The results showed that the optimum temperature is 40℃and the proper pH 7.0.In these circumstances,citric arid can significantly inhibit the activities of PPO,while ascorbic acid is also effective,but sodium sulfite andβ-cyclodextrin are less effective.Tannin has the biggest affinity with PPO among three substrates tested.

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What this paper is about

Spectrophotometer method was applied in this experiment to study the characteristics of polyphenol oxidase(PPO) in pomegranates.This article also analyzed the effects of citric acid,ascorbic acid,sodium sulfite andβ-cyclodextrin on PPO. The results showed that the optimum temperature is 40℃and the proper pH 7.0.In these circumstances,citric arid can significantly inhibit the activities of PPO,while ascorbic acid is also effective,but sodium sulfite andβ-cyclodextrin are less effective.Tannin has the biggest affinity with PPO among three substrates tested.

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Available abstract

Spectrophotometer method was applied in this experiment to study the characteristics of polyphenol oxidase(PPO) in pomegranates.This article also analyzed the effects of citric acid,ascorbic acid,sodium sulfite andβ-cyclodextrin on PPO. The results showed that the optimum temperature is 40℃and the proper pH 7.0.In these circumstances,citric arid can significantly inhibit the activities of PPO,while ascorbic acid is also effective,but sodium sulfite andβ-cyclodextrin are less effective.Tannin has the biggest affinity with PPO among three substrates tested.

Key concepts: Polyphenol oxidase, Citric acid, Browning, Chemistry, Ascorbic acid, Sulfite, Tannin, Sodium sulfite

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