Optimization of polyphenols extraction from grape seeds by response surface methodology

To optimize the process for polyphenols extraction from grape seeds,acetone was chosen as the best extraction solvent for this research.Then a series of single-factor experiments were made to investigate the effects of acetone concentration,extraction duration,temperature and times on polyphenols yield,and based on this,a 19-run response surface design was generated by the Design-Expert software according to the central composite design principle and experimental data obtained were subjected to quadratic regression analysis to create a mathematical model describing polyphenols extraction.The optimal extraction conditions were found to be: acetone concentration of 72% for an extraction duration of 32 min at 65 ℃ 4 times.Under these conditions,the yield of polyphenols was 2.892 mg/mL.