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Quantitative determination of arachidonic acid from microbial oil by gas chromatography

Dong Hongzhen, Tang Peiping, XU Jianzhong, Chen Xu

Open publisher page 2 citations

Abstract

A gas chromatography(GC) method for the determination of arachidonic acid(AA) in microbial oil was developed.Capillary column HP-INNOWAX with FID was used for detection.The parameters for analyzing esterfied AA were optimized and the method established was proved to be accurate and precise.The linearity range for methyl arachidonate was 0-1.00 mg/mL and the linear correlation coefficient was 0.999 6,the detection limit was 0.000 5 mg/mL,the relative standard deviation(RSD)was 1.49% and the recovery rate was 95.86%-105.32%.The linearity range for ethyl arachidonate was 0-1.23 mg/mL and the linear correlation coefficient was 0.999 9,the detection limit was 0.003 0 mg/mL,the RSD was 0.60% and the recovery rate was 97.79%-103.61%.

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What this paper is about

A gas chromatography(GC) method for the determination of arachidonic acid(AA) in microbial oil was developed.Capillary column HP-INNOWAX with FID was used for detection.The parameters for analyzing esterfied AA were optimized and the method established was proved to be accurate and precise.The linearity range for methyl arachidonate was 0-1.00 mg/mL and the linear correlation coefficient was 0.999 6,the detection limit was 0.000 5 mg/mL,the relative standard deviation(RSD)was 1.49% and the recovery rate was 95.86%-105.32%.The linearity range for ethyl arachidonate was 0-1.23 mg/mL and the linear correlation coefficient was 0.999 9,the detection limit was 0.003 0 mg/mL,the RSD was 0.60% and the recovery rate was 97.79%-103.61%.

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Available abstract

A gas chromatography(GC) method for the determination of arachidonic acid(AA) in microbial oil was developed.Capillary column HP-INNOWAX with FID was used for detection.The parameters for analyzing esterfied AA were optimized and the method established was proved to be accurate and precise.The linearity range for methyl arachidonate was 0-1.00 mg/mL and the linear correlation coefficient was 0.999 6,the detection limit was 0.000 5 mg/mL,the relative standard deviation(RSD)was 1.49% and the recovery rate was 95.86%-105.32%.The linearity range for ethyl arachidonate was 0-1.23 mg/mL and the linear correlation coefficient was 0.999 9,the detection limit was 0.003 0 mg/mL,the RSD was 0.60% and the recovery rate was 97.79%-103.61%.

Key concepts: Chromatography, Correlation coefficient, Detection limit, Chemistry, Linear correlation, Gas chromatography, Relative standard deviation, Recovery rate

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