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2015•Shengtaixue zazhiRequires access

RAPD analysis on genetic diversity of wild populations of Carya cathayensis in southern mountainous area of Anhui Province

Guo Chuan-yo

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Abstract

Genetic diversity and genetic differentiation were examined in four wild populations of Carya cathayensis in southern mountainous area of Anhui Province using random amplified polymorphic DNA( RAPD) markers. As analyzed by POPGENE software,a total of 139 discernible loci were obtained from all populations using 15 random primers,62. 59% of which( 87 fragments) were polymorphic loci,with an average percentage of polymorphic loci as 43. 35% for all populations. At species level,Nei's genetic diversity( H = 0. 124) and Shannon's information index( I = 0. 208) indicated that C. cathayensis had a relatively low genetic diversity. The higher value of differentiation( Gst= 0. 417) and the lower value of gene flow( Nm= 0. 700) showed that the geographic isolation has an obvious influence among populations.

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Genetic diversity and genetic differentiation were examined in four wild populations of Carya cathayensis in southern mountainous area of Anhui Province using random amplified polymorphic DNA( RAPD) markers. As analyzed by POPGENE software,a total of 139 discernible loci were obtained from all populations using 15 random primers,62. 59% of which( 87 fragments) were polymorphic loci,with an average percentage of polymorphic loci as 43. 35% for all populations. At species level,Nei's genetic diversity( H = 0. 124) and Shannon's information index( I = 0. 208) indicated that C. cathayensis had a relatively low genetic diversity. The higher value of differentiation( Gst= 0. 417) and the lower value of gene flow( Nm= 0. 700) showed that the geographic isolation has an obvious influence among populations.

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Available abstract

Genetic diversity and genetic differentiation were examined in four wild populations of Carya cathayensis in southern mountainous area of Anhui Province using random amplified polymorphic DNA( RAPD) markers. As analyzed by POPGENE software,a total of 139 discernible loci were obtained from all populations using 15 random primers,62. 59% of which( 87 fragments) were polymorphic loci,with an average percentage of polymorphic loci as 43. 35% for all populations. At species level,Nei's genetic diversity( H = 0. 124) and Shannon's information index( I = 0. 208) indicated that C. cathayensis had a relatively low genetic diversity. The higher value of differentiation( Gst= 0. 417) and the lower value of gene flow( Nm= 0. 700) showed that the geographic isolation has an obvious influence among populations.

Key concepts: RAPD, Genetic diversity, Gene flow, Biology, Botany, Genetic variation, Genetics, Gene

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RAPD analysis on genetic diversity of wild populations of Carya cathayensis in southern mountainous area of Anhui Province — Research Paper | ScholarLens