Relationship of Methylation of the Runx3 Gene and Expression of Protein in Laryngeal Carcinoma

Objective To investigate the relationship between methylation and the expression of Runx3 in promoter region and and its possible relationship with the clinicopathological features of human laryngeal carcinoma.Methods Using immunohistochemistry technique,specimens from 55 patients of carcinoma of larynx(tumor tissues,adjacent normal tissues)were detected for their expression of the Runx3 gene.Meanwhile,Methylation-specific PCR was used to detect methylation of Runx3 at promoter region.Results The expression of Runx3 protein in carcinoma of Larynx was down-regulated than that in adjacent normal tissues samples(P0.05).No methylation of Runx3 promoter was found in adjacent normal tissues samples,but was detected in 32 cases(58.2%)of 55 carcinoma of larynx.The rate of methylation of Runx3 promoter in carcinoma of larynx was higher than that of in adjacent normal tissues(P0.05),and it was not related to the gender、clinical classification、tumor pathological grade and lymph node metastasis(P0.05).There was correlation between methylation of Runx3 gene promoter and expression of its protein(r=-0.7092,P0.01).Conclusion Hypermethylation may cause the Runx3 gene inactivation in human carcinoma of Larynx.Methylation of runx3 promoter maybe correlated with genesis of carcinoma of larynx.