2010China Medical HeraldRequires access

The relationship between semen quality and the level of seminal plasma elastase and leukocytes in male infertility

Xiaoxia Zhang

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Abstract

Objective:To explore the relationship between elastase levels,leukocyte numbers and seminal plasma and semen quality of patients with male infertility.Methods:Enzyme-linked immunosorbent assay(ELISA)was used to detect 137 cases of patients with male infertility in seminal plasma elastase levels,seminal plasma interleukin was quantitatively determined by peroxidase staining,semen quality was analyzed by using CASAS-QH-Ⅲ computer-aided semen analyzer.Results:Elastase level abnormal group showed significant difference in semen pH,sperm density,the percentage of a level sperm and sperm motility compared with those of the normal group(P0.05);inapparent infection elastase level group showed significant difference in semen group pH,the percentage of b level sperm and sperm motility compared with those of the confirmed infected group(P0.05);the levels of seminal plasma and leukocyte elastase quantitative were positively correlated(rs=0.561,P0.01);infertile patients with normal leukocyte content group(1×106/ml)showed significant difference in semen pH,the percentage of a level sperm and sperm motility compared with those of the abnormal group(≥1×106/ml)(P0.05).Conclusion:Elastase levels and leukocyte numbers in seminal plasma enzyme may be closely related to semen quality and infertility.

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What this paper is about

Objective:To explore the relationship between elastase levels,leukocyte numbers and seminal plasma and semen quality of patients with male infertility.Methods:Enzyme-linked immunosorbent assay(ELISA)was used to detect 137 cases of patients with male infertility in seminal plasma elastase levels,seminal plasma interleukin was quantitatively determined by peroxidase staining,semen quality was analyzed by using CASAS-QH-Ⅲ computer-aided semen analyzer.Results:Elastase level abnormal group showed significant difference in semen pH,sperm density,the percentage of a level sperm and sperm motility compared with those of the normal group(P0.05);inapparent infection elastase level group showed significant difference in semen group pH,the percentage of b level sperm and sperm motility compared with those of the confirmed infected group(P0.05);the levels of seminal plasma and leukocyte elastase quantitative were positively correlated(rs=0.561,P0.01);infertile patients with normal leukocyte content group(1×106/ml)showed significant difference in semen pH,the percentage of a level sperm and sperm motility compared with those of the abnormal group(≥1×106/ml)(P0.05).Conclusion:Elastase levels and leukocyte numbers in seminal plasma enzyme may be closely related to semen quality and infertility.

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Available abstract

Objective:To explore the relationship between elastase levels,leukocyte numbers and seminal plasma and semen quality of patients with male infertility.Methods:Enzyme-linked immunosorbent assay(ELISA)was used to detect 137 cases of patients with male infertility in seminal plasma elastase levels,seminal plasma interleukin was quantitatively determined by peroxidase staining,semen quality was analyzed by using CASAS-QH-Ⅲ computer-aided semen analyzer.Results:Elastase level abnormal group showed significant difference in semen pH,sperm density,the percentage of a level sperm and sperm motility compared with those of the normal group(P0.05);inapparent infection elastase level group showed significant difference in semen group pH,the percentage of b level sperm and sperm motility compared with those of the confirmed infected group(P0.05);the levels of seminal plasma and leukocyte elastase quantitative were positively correlated(rs=0.561,P0.01);infertile patients with normal leukocyte content group(1×106/ml)showed significant difference in semen pH,the percentage of a level sperm and sperm motility compared with those of the abnormal group(≥1×106/ml)(P0.05).Conclusion:Elastase levels and leukocyte numbers in seminal plasma enzyme may be closely related to semen quality and infertility.

Key concepts: Semen, Sperm, Andrology, Elastase, Semen quality, Infertility, Male infertility, Sperm motility

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