Analysis of dynamic changes of interleukin 1β and prostaglandin E2 in smoke inhalation injury repair model

AIM:To investigate the dynamic changes of interleukin 1β(IL-1β) and prostaglandin E2(PGE2) levels in smoke inhalation injury repair in New Zealand white rabbits.METHODS:New Zealand white rabbits(n=36) were randomly divided into normal control group,and 1,4,7,14 and 21 d after smoke inhalation injury groups,with 6 animals in each group.ELISA was used to measure the levels of IL-1β and PGE2.The smoke inhalation injury repair model was established and the pathologic changes of smoke inhalation on trachea and lung tissues were observed by light microscope.RESULTS:The pathologic changes showed that mainly inflammatory reaction(injury stage) was seen in 1,4 and 7 d after injury groups and mainly repair(repair stage) was observed in 14 and 21 d after injury groups.The results of ELISA showed that IL-1β concentration was upregulated in 1 d group[(196.2±67.8) ng/L] after smoke inhalation injury,higher than that in normal group[(7.1±0.2) ng/L,P0.05].IL-1β concentration peaked at day 7[(398.5±161.4) ng/L] and then began to decrease.IL-1β concentration was the lowest at day 21[(9.3±3.7) ng/L] and no significant difference was observed compared with that in normal group(P0.05).PGE2 concentrations were upregulated gradually from 1 d[(4.4±1.0) μg/L] to 14 d[(120.9±61.1) μg/L] following smoke inhalation injury.PGE2 concentrations peaked at day 14 and then began to decrease,with significant difference from those in normal control group [(4350±1021) ng/L,P0.05].PGE2 concentration was the lowest at day 21[(6423±2812) ng/L],with no significant difference from that in normal group(P0.05).CONCLUSION:The dynamic changes of IL-1β and PGE2 levels in smoke inhalation injury repair may indicate the different stages(injury stage or repair stage) of smoke inhalation injury repair process.IL-1β and PGE2 levels may play some important role in the smoke inhalation injury repair.