The recombinant Expression and clinical application of Autoantigen gp210

Objective To express gp210 recombinant fusion protein in E.coli and apply to clinical assay.Methods The cDNA encoding gp210 was obtained by RT-PCR,confirmed by DNA sequencing,subcloned into the bacterial expression plasmid pET28a(+) and then transformed into E.coil.BL21(DE3) to express the recombinant fusion protein induced by IPTG.ELISA was established to detected anti-gp210 antibody in PBC patients.Results The recombinant fusion protein was 69kDa as expected and exhibited the antigenicity of gp210 by Western-blot.The positive ratio of anti-gp210 antibody in PBC was 40.5%.The concentrations of IgM in patients with anti-gp210 antibody were significantly higher than patients without anti-gp210 antibody.Conclusion The recombinant gp210 fusion protein could be used for monitoring the course of Primary biliary cirrhosis.