Optimization of cellulose productiong by solid-state fermentation with Trichoderma viride 95106

Using straw powder and wheat bran as raw material,the influence of the ratio of straw flour to wheat bran,the ratio of solid-liquid,initial pH value,nitrogen source and concentration,fermentation temperature and time on cellulose production by solid-state fermentation of Trichoderma viride were studied in this paper.The optimal fermentation conditions were obtained as follows: the ratio of straw flour to wheat bran 15∶5,the ratio of solid-water l∶5,initial pH value 6.0,urea concentration 0.25%,fermentation temperature 28℃ and fermentation time 72h,Under these conditions,the highest enzyme production was obtained.The filter paper activity and endonuclease activity were increased by 121% and 72.6% compared with those before optimization,respectively.The results could provide some reference for industrialized production of cellulase with high efficiency and low cost.