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2009•Anhui nongye kexueRequires access

Study on Isolation and Characterization of a DDT Degradation Bacterium Strain DH-7

Hongquan Li

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Abstract

[Objective]The purpose of this research was to study biological characteristics,degradation characteristics and the optimization of fermentation conditions of DDT degradation bacterium.[Method] A bacterium strain DH-7 having capability of degrading DDT was isolated from the DDT contaminated soil of a chemical factory.The characteristics of DH-7 were studied.[Result]Based on the phenotype,physiological and biochemical characteristics,and the phylogenetic analysis of 16S rDNA sequence,the strain DH-7 was identified preliminarily as Pseudomonas aeruginosa.This strain could degrade DDT with degradation efficiency of 73.6% in 10 days.The degradation efficiency was up to 81.4% after the conditions of culture was optimized.[Conclusion]This study will provide the references for bioremediation of contaminated soil by DDT.

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[Objective]The purpose of this research was to study biological characteristics,degradation characteristics and the optimization of fermentation conditions of DDT degradation bacterium.[Method] A bacterium strain DH-7 having capability of degrading DDT was isolated from the DDT contaminated soil of a chemical factory.The characteristics of DH-7 were studied.[Result]Based on the phenotype,physiological and biochemical characteristics,and the phylogenetic analysis of 16S rDNA sequence,the strain DH-7 was identified preliminarily as Pseudomonas aeruginosa.This strain could degrade DDT with degradation efficiency of 73.6% in 10 days.The degradation efficiency was up to 81.4% after the conditions of culture was optimized.[Conclusion]This study will provide the references for bioremediation of contaminated soil by DDT.

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Available abstract

[Objective]The purpose of this research was to study biological characteristics,degradation characteristics and the optimization of fermentation conditions of DDT degradation bacterium.[Method] A bacterium strain DH-7 having capability of degrading DDT was isolated from the DDT contaminated soil of a chemical factory.The characteristics of DH-7 were studied.[Result]Based on the phenotype,physiological and biochemical characteristics,and the phylogenetic analysis of 16S rDNA sequence,the strain DH-7 was identified preliminarily as Pseudomonas aeruginosa.This strain could degrade DDT with degradation efficiency of 73.6% in 10 days.The degradation efficiency was up to 81.4% after the conditions of culture was optimized.[Conclusion]This study will provide the references for bioremediation of contaminated soil by DDT.

Key concepts: Bioremediation, Strain (injury), Degradation (telecommunications), Bacteria, 16S ribosomal RNA, Isolation (microbiology), Fermentation, Bacterial strain

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