Effect of Zinc, Copper, Calcium on carbonic anhydrase and fluorescence of plants

We Used different concentrations of zinc,copper,calcium to deal with three dominant tree species ———Pinus armandi,Populus yunnanensis and White oak from Caohai of Guizhou,determinated the carbonic anhydrase activity,chlorophyll content and fluorescence parameters of those plants and analyzed their impacts on the photosynthetic carbon sequestration capacity.The results showed that:(1) With the increase of the concentration of zinc,there was almost no chang in carbonic anhydrase activity of P.armandii,but the carbonic anhydrase activity of P.yunnanensis and White oak increased,the chlorophyll content and fluorescence parameters of the three species also increased.(2) With the increase of the concentration of copper,there was almost no chang in carbonic anhydrase activity of P.armandii,but the carbonic anhydrase activity of P.yunnanensis and White oak increased first and then decreased,the chlorophyll content and the parameter values of Y(Ⅱ),ETR,qP,Fv/Fm of the three species also increased at first,then decreased.However,the qN of the three species decreased at first,then increased.(3) With the increase of the concentration of calcium,the carbonic anhydrase activity,chlorophyll content and the parameter values of Y(Ⅱ),ETR,qP,Fv/Fm of the three species increased at first,then decreased.There was almost no chang in the value of qN.Therefore,the application of zinc,copper,calcium can improve the carbonic anhydrase activity and photosynthetic carbon sequestration ability of plant,which is significant to the carbon sequestration ability optimization of the plant in carst areas.