2007•Shandong yiyaoRequires access
Effects of all-trans retinoic acid on growth of esophageal cancer cells and expression of survivin gene
Ruilin Wang
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Abstract
[Objective]To observe inhibitory effects of all-trans retinoic acid(ATRA) on growth of esophageal cancer cells in vitro and expression of survivin gene.[Methods]The inhibition rates of proliferation were detected by MTT assay.RT-PCR and flow cytometry were used to detect the expression of survivin gene and the apoptosis rates.[Results]After treatment with ATRA,the proliferation of EC-9706 was inhibited in dose-and time-dependent manner,and the apoptosis rates of EC-9706 cells were increased significantly.The expression of survivin mRNA was decreased obviously in EC-9706 cells treated with ATRA.[Conclusion]ATRA can inhibit esophageal cancer cells growth, induce its apoptosis.Down-regulation of survivin gene probably contributes to the apoptosis of esophageal cancer cells induced by ATRA.