Apoptosis in human lung carcinoma cell line A549 induced by recombinant caspase-3

Objective To investigate the apoptosis in human lung carcinoma cell line A549 induced by recombinant Caspase 3 gene.Methods The expression vector pEGFP C1 with caspase 3 gene was constructed and transferred into the A549 cell line.The expression level of caspase 3 gene in A549 cell line was detected by RT PCR method and immunohistochemical staining.The cell morphological changes were observed under fluorescent microscopy and electron microscopy.The cell survival rate was assayed by MTT method.Results The expression of caspase 3 gene in A549 cells was detectable by RT PCR method and immunohistochemical staining 48 h after transfection.The cell morphological observation and MTT showed the apoptosis in A549 cell line and the cell growth curve declined 24 48 h after transfection.Conclusion Recombinant Caspase 3 gene can effectively induce A549 cells to apoptosis.