Experimental study of the effect of RNAi silencing STAT3 gene expression on the growth of bladder cancer cells

Objective To explore the effect of signal transducers and activators of transcription 3 (STAT3) silenced by RNA interference (RNAi) technique on bladder cancer cells T24 and 5637.Methods Targeted at STAT3 mRNA sequence, three pairs of DNA templates encoding small interference RNA (siRNA) were designed and synthesized. The recombinant plasmid pGenesil-1-shRNA-STAT3 was constructed and transfected into T24 and 5637 cells. Semi-quantitative RT-PCR and Western blot were applied to detecting STAT3 gene expression. Methyl thiazolyl tetrazolium (MTT) assay and flow cytometry were applied to detecting the changes of cell proliferation and cycle.Results pGenesil-1-shRNA-STAT3 was successfully constructed, and transfected into T24 and 5637 cells. Semi-quantitative RT-PCR and Western blot analysis demonstrated that pGenesil-1-shRNA-STAT3 could significantly inhibit the expression of STAT3 in T24 and 5637 cells;MTT and FCM results showed that it could suppress the growth of T24 and 5637 cells.Conclusion pGeneSil-1-shRNA-STAT3 transfection into T24 and 5637 cells can significantly inhibit STAT3 expression,and while suppress the growth and proliferation of T24 and 5637 cells.