Eukaryotic expression of the VP1 gene of foot-and-mouth disease virus type O and characterization of expressed fusion protein

According to the published VP1 gene sequence,one pair of primers with SacⅠ and HindⅢ endonuclease sites was designed.A VP1 gene in the plasmid pMD18-T-VP1 was subcloned into an eukaryotic vector pBlueBacHis2A,and the recombinant plasmid pBlueBacHis2A-VP1(633bp) was constructed successfully.The plasmid pBlueBacHis2A-VP1(633bp) and Bac-N-Blue~(TM) DNA were co-transfected into sf9 cells.After screening the plaques,sf9 cells were infected with the recombinant virus.The target protein of 32.6ku in size was obtained.Dot-ELISA analysis showed that the protein had good antigenicity.