Preparation and Evaluation of Rat T_(10) Spinal Hemisection Model

Objective A spinal cord injury model was prepared by hemisection T10 spinal cord,to investigate the pathophysiology,and provide a basis for the mechanism and treatment of acute spinal cord injury. Methods The 24 adult female SD rats were randomly divided into two groups: spinal cord injury group( 12 rats) and sham group( 12 rats). Rats in spinal cord injury group were bitten off the spinous and the corresponding lamina,transversed half off T10 spinal while in the sham group,rates were only with laminectomy. BBB motor function score was assessed at 1,3,5,7,14,21,28 days postoperatively. At the same time,N1 and P1 wave latency of motor evoked potentials and somatosensory evoked potentials were recorded at 7,14,21,28 days postoperatively,to assess the integrity of nerve pathways. Results Compared with the sham group,the spinal cord injury groups had lower BBB postoperative motor function score( P 0. 05) at each time point postoperatively. The results of neurophysiological test showed that there were significant differences in motor evoked potentials and somatosensory evoked potential latencies between spinal cord injury group and the sham group( P 0. 05). Conclusion The method of acute spinal cord injury model established in this study is easy to copy and can be used for further spinal cord injury research.