Comparison of effects of icaritin and anhydroicaritin on lymphocytes

Mouse lymphocytes were isolated.The drug toxicology of different concentrations of AHI/ ICA on lymphocytes was measured by CCK-8 assay.The expression of CD69 and CD25,which were the markers of activation of T lymphocytes,was measured by FCM combined with two-color immunofluorescent staining of cell surface antigen.The proliferation of T lymphocytes was measured by FCM combined with live dye CFSE.The cell cycle of T lymphocytes was measured by FCM.The Calcium influx of T lymphocytes was measured by FCM’s time function.CBA kit was used to detect the production of cytokines of lymphocytes stimulated by con A.AHI and ICA decreased proportions of CD69 and CD25 on con A-stimulated lymphocytes in a dose-dependent manner.Both reduced the proliferation of T lymphocytes,and the influx of Calcium.AHI and ICA caused a G0 / G1 cell cycle arrest on con A-activated lymphocytes.Furthermore,both downregulated the secretion of cytokines(IL-6,IL-10,IFN-γ,TNF,IL12p70) of con A-activated lymphocytes.Furthermore,ICA exhibited more immunosuppressive effects than AHI on mouse T lymphocytes.