Inhibition Effect of Protocatechuik Acid on Tyrosinase Acitivity and Melanin Content in Mouse Melanoma B16 Cell

Tyrosinase(EC 1.14.18.1)plays an important role in melanogenesis,thus tyrosinase inhibitors have multiple potential applications.Screening safe inhibitors from natural herbal medicines is of important significances.In this study,the inhibitory effect of protocatechuik acid,an activite ingredient of Chinese traditional Medicinces,on melanin content and tyrosinase activity from mouse melanoma cell(B16cell)were studied.The results showed that protocatechuik acid can decrease 60% of melanin content in mouse B16cell.Further research demonstrated that protocatechuik acid has significant inhibitory effect on crude tyrosinase from both mouse B16cell and co-culture system.It can significantly decrease tyrosinase activity.The IC50of protocatechuik acid on crude tyrosinase is about 160μmol/L.The inhibition effects were related with time of duration and concentration.The inhibition rate was about 88%after treated with 10μmol/L protocatechuik acid for 54h.The study provided a fundamental framework for the research and application of protocatechuik acid as tyrosinase inhibitor.