Genetic Diversity of Fontinalaceae by ISSR Analysis

The genetic diversity of 13 species Fontinalaceae collected in diferent regions was studied using ISSR marker technique method. The results showed that 526 clear bands were amplified by 7 stable and highly reproducible primers of which 518 were polymorphic. the percentage of polymorphic bands reached 98.47%. The result of analyzed by the software POPGENE-32 showed that number of alleles Na=1.7363, efective number of alleles Ne=1.3062. Nei gene diversity and Shannon information index were 0.201 5 and 0.321 1. It showed that the geographical distribution was very obvious. The coefcient of gene diferentiation Gst, genetic distance D and genetic identity I of 13 samples were 0.025 3,0.007 1 and 0.054 7, indicating there was a certain gene flow among diferent species of Fontinalaceae and the genetic variation was mainly within species compared with interspecies. Cluster analysis among 13 samples genetic relationship using software NTSYS2.1 showed that these samples were divided into there groups. In addition,there were some correlation between genetic distance and geographic distance among the samples.