2010•Clinical Medicine & EngineeringRequires access

The Study of Best Cultivated Condition in Silver-staining

Zhong Yun

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Abstract

Objective To investigate the best cultivated condition of bacillus subtilis in silver-staining. Methods Bacillus subtilis was cultivated with 30 ℃,35 ℃ nutrient agar,blood agar and bouillon for 6 ~ 12 h,and taken to smear at 6,8,10,12 h each. Bacterial flagella was observed with micro after argentation. Results Many integrity flagella and clean vision were observed after raising bacillus subtilis in 30 ℃ nutrient agar for 8 ~ 10 h and staining. Conclusion To cultivate the bacillus subtilis in 30 ℃ nutrient agar for 8 ~ 10 h and stain have the best result.

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Objective To investigate the best cultivated condition of bacillus subtilis in silver-staining. Methods Bacillus subtilis was cultivated with 30 ℃,35 ℃ nutrient agar,blood agar and bouillon for 6 ~ 12 h,and taken to smear at 6,8,10,12 h each. Bacterial flagella was observed with micro after argentation. Results Many integrity flagella and clean vision were observed after raising bacillus subtilis in 30 ℃ nutrient agar for 8 ~ 10 h and staining. Conclusion To cultivate the bacillus subtilis in 30 ℃ nutrient agar for 8 ~ 10 h and stain have the best result.

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Available abstract

Objective To investigate the best cultivated condition of bacillus subtilis in silver-staining. Methods Bacillus subtilis was cultivated with 30 ℃,35 ℃ nutrient agar,blood agar and bouillon for 6 ~ 12 h,and taken to smear at 6,8,10,12 h each. Bacterial flagella was observed with micro after argentation. Results Many integrity flagella and clean vision were observed after raising bacillus subtilis in 30 ℃ nutrient agar for 8 ~ 10 h and staining. Conclusion To cultivate the bacillus subtilis in 30 ℃ nutrient agar for 8 ~ 10 h and stain have the best result.

Key concepts: Bacillus subtilis, Nutrient agar, Agar, Staining, Agar plate, Bacillus (shape), Gram staining, Food science

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