Establishment of double PCR method for detecting food-borne pathogenic bacteria about Salmonella and Proteus mirabilis

Objective: To establish a multiplex polymerase chain reaction( PCR) assay for simultaneous identification of Proteus mirabilis and Salmonella. Methods: Based on the gene sequences of positive adjustment factor R gene of Urease( ureR) in Proteus mirabilis and conservative invasive antigen gene( invA) in Salmonella,two pairs of primer were designed. Its double PCR detection method was established,and the reaction conditions were optimized. Results: Under the optimized conditions,the anticipated PCR products were 374 bp and 724 bp,respectively,and were highly specific,and the minimum detection limit was 104cfu / ml. Conclusion: A rapid,specific and sensitive multiplex PCR system has been established and it is valuable for the diagnosis and the monitoring for Proteus mirabilis and Salmonella.