RAPD and SCAR molecular markers for male trait in Caric papaya

The random amplified polymorphic DNA (RAPD) technique was used to determine the male trait of Carica papaya L.. 214 10-mer primers were tested. A male-associated fragment was generated with Z18 primer. The marker fragment, named Z18-1000 existed in all male plants but not in the female and hermaphrodite plants so far analyzed. The fragment was cloned and sequenced. Four primers were designed based on the sequence to transform RAPD marker into SCAR marker. The RAPD marker was successfully converted into a SCAR marker, which was designated SD1000.