Construction of TNNI2 Mutation Transgenic Mice

Objective To construct the animal model of TNNI2 mutation transgenic mice.Methods pEGFP-tnni2(p.K175del) was constructed,linearized and purified,then microinjected into fertilized mouse eggs.These eggs were transplanted into the pseudopregant mice.The genotype of transgenic founders were identified by PCR and Southern blot.The expressions of tnni2 transcript in the tissues of the transgenic mice were detected by RT-PCR.Result Transgenic BDF1 mice were obtained by microinjection.PCR and Southern blot results showed 4 of 23 mice were integrated.Two of the founders were positive by RT-PCR.Conclusion TNNI2 mutation transgenic mice were successfully established by pronuclear microinjection.