The effects of estradiol benzoate on the number and distribution of mast cells in the reproductive organs of mice

To investigate the effects of estradiol benzoate(E2) on the number and distribution of mast cells in mice reproductive organs,fifty mice(four-week old) were randomly divided into five groups,namely two control groups and three treatment groups,with 10 mice(half of each sex) in each group.Mice had been administered(0.05,0.5,5mg·kg-1·d-1 with E2 by celiac injection for 7 days.The samples of testis,epididymis,ovary,and the uterus were then collected by surgical operation.The tissues samples were fixed in 4% paraformaldehyde in phosphate-buffered saline for 24h and then processed to paraffin wax.Five-micron-thick sections were cut for mast cell detection.The distribution and number of mast cells in the reproductive organ of mice were determine by using modified toluidine blue(MTB) staining.The results showed as follows:0.05mg·kg-1·d-1 dose of E2 administrated significantly increase uterus mast cells,but 0.5mg·kg-1·d-1 and 5mg·kg-1·d-1 dose of E2 significantly decreased uterus mast cell.All doses of E2 administrated significantly increased ovary mast cells.0.05mg·kg-1·d-1 and 0.5mg·kg-1·d-1 dose of E2 administrated increased the mast cell number in the testes and epididymis,but 5mg·kg-1·d-1 dose of E2 significantly decreased the mast cells in testicle.