Evaluation the ovariectomized rats as animal model for osteoporosis viva trial

Objectives: To evaluate the ovariectomized rats after three months as animal model for osteoporosis study. Methods: Eight female Sprague-Dawley (SD) rats aged 24 weeks from sham and OVX group respectivityly were anesthetized. Blood samples were withdrawn to assess biochemical markers of bone metabolism. At sacrifice, the femora and tibia were separated, cleaned of soft tissues. MicroCT scanning, bone histomorphometric analysis and histopathological observation, biomechanical testing were progressed gradually. The data were statistically analyzed by SPSS13.0. Results: Serum ALP activity and osteocalcin levels elevated significantly in OVX group than Sham group (P0.05). Trabecular bone volume and area, trabecular thickness, trabecular number, BMC and BMD of OVX group all were reduced than those of Sham group (P0.05). Trabecular separation increased significantly. Compared with sham group, elastical load of OVX group increased (P0.05). Maximal stress and elastical stress deceased significantly. Conclusions: Evaluated by multi-methods, after three months, the OVX rats are in osteoporotic status. Bone mass is reduced. Trabecular bone architecture has changed. And bone biomechanical performance is worsening.