Effect of β-amyloid peptide 25-35 on the hippocampal and septal neurons in vitro

Objective To explore the morphological effects of β-amyloid peptide 25-35 (Aβ 25-35 ) on hippocampal and septal cholinergic neurons. Methods The primary culture hippocampal and septal neurons were exposed to 5umol/L Aβ 25-35 for 48h and 10umol/L Aβ 25-35 for 24 h. The survival of neurons in the two situations was compared. The immunohistochemistry and telemicroscope methods were used to show the morphological change of branches and nuclear. Results The survival rate of hippocampal and septal neurons were decreased after treatment. Aβ 25-35 damaged the branch of the neuron and induced the morphological change of apoptosis. Conclusions Aβ 25-35 has toxic effects which was related to its concentration and time on the hippocampal and cholinergic neuron, and it could damage the branch of neuron and induce the pathologic change of and apoptosis.