On Technique for Saccharification of Dioscorea Zingiberens Starch by Double Enzyme Method

For the technological conditions for saccharification of dioscorea zingiberens starch by double enzyme method.Through orthogonal optimizing experiments and simple factor experiments,the optimized technological condition for liquefaction and saccharification of dioscorea zingiberens starch was obtained.When thick raw starch liquid of dioscorea zingiberens was 22.5%,the best liquefying conditions were as follows:pH7.0,temperature 95℃,the amount of α-amylase 12U/g starch;the best scarifying conditions were as follows:pH5.0,temperature 55℃,the amount of glucoamylase 180U/g starch,the amount of alcohol enzyme 0.05 % mL.mL-1,the time of scarifying 48h.Under the optimized technological conditions for liquefaction and saccharification of dioscorea zingiberens starch,the raw starch conversion rate reached 96.02 %,the glucose receives rate reached 106.58%,the content of dextrose of sugar fluid reached 23.98%.