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Activation of Cell Factors of EGF mRNA after Middle Cerebral Artery Occlusion in Rats and Treatment with Tongxinluo

Lixin Wang

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Abstract

Objective:To explore the expression of epidermal growth factor(EGF)mRNA on cerebral ischemia-reperfusion injury in rats brain tissues and the effect by Tongxinluo.Methods:VD models of rat(MCAO)were perfused with Tongxinluo,detecting the changes of neural stem cell proliferation and differentiation related cell factors of EGF mRNA after ischemia-reperfusion injury on 5th,7th,14th,21st,30th day by means of reverse transcriptase polymerase chain reaction(RT-PCR).Results:EGF mRNA of ischemia-reperfusion model was expressed in different periods,beginning to enhance expression on the third day,highest on the fifth day.EGF mRNA began to express enhanced after giving Tongxinluo on the third day,on 5th,7th,14th,21st and 30th day PCR expression was higher than that in the control group.Conclusion:EGF mRNA expression in ischemia brain area is enhanced and Tongxinluo can strengthen the expression and then may induce neural stem cell proliferation.

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Objective:To explore the expression of epidermal growth factor(EGF)mRNA on cerebral ischemia-reperfusion injury in rats brain tissues and the effect by Tongxinluo.Methods:VD models of rat(MCAO)were perfused with Tongxinluo,detecting the changes of neural stem cell proliferation and differentiation related cell factors of EGF mRNA after ischemia-reperfusion injury on 5th,7th,14th,21st,30th day by means of reverse transcriptase polymerase chain reaction(RT-PCR).Results:EGF mRNA of ischemia-reperfusion model was expressed in different periods,beginning to enhance expression on the third day,highest on the fifth day.EGF mRNA began to express enhanced after giving Tongxinluo on the third day,on 5th,7th,14th,21st and 30th day PCR expression was higher than that in the control group.Conclusion:EGF mRNA expression in ischemia brain area is enhanced and Tongxinluo can strengthen the expression and then may induce neural stem cell proliferation.

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Available abstract

Objective:To explore the expression of epidermal growth factor(EGF)mRNA on cerebral ischemia-reperfusion injury in rats brain tissues and the effect by Tongxinluo.Methods:VD models of rat(MCAO)were perfused with Tongxinluo,detecting the changes of neural stem cell proliferation and differentiation related cell factors of EGF mRNA after ischemia-reperfusion injury on 5th,7th,14th,21st,30th day by means of reverse transcriptase polymerase chain reaction(RT-PCR).Results:EGF mRNA of ischemia-reperfusion model was expressed in different periods,beginning to enhance expression on the third day,highest on the fifth day.EGF mRNA began to express enhanced after giving Tongxinluo on the third day,on 5th,7th,14th,21st and 30th day PCR expression was higher than that in the control group.Conclusion:EGF mRNA expression in ischemia brain area is enhanced and Tongxinluo can strengthen the expression and then may induce neural stem cell proliferation.

Key concepts: Medicine, Epidermal growth factor, Ischemia, Messenger RNA, Neural stem cell, Stem cell, Reverse transcription polymerase chain reaction, Reperfusion injury

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Activation of Cell Factors of EGF mRNA after Middle Cerebral Artery Occlusion in Rats and Treatment with Tongxinluo — Research Paper | ScholarLens