Study of methyl salicylate glycosides in acute pleurisy model in rats

Aim To study the anti-inflammatory effect and mechanism of methyl salicylate glycosides(DL0309) in carrageenan-induced acute pleurisy model in rats,and to provide the pharmacology mechanism for the effects and clinical use of DL0309.Methods Forty-eight male rats were divided into 6 groups: normal group,pleurisy model group,dexamethasone group(positive drug),DL0309 low dose(150 mg·kg-1),medium dose(300 mg·kg-1),and high dose(600 mg·kg-1) groups.The acute pleurisy model was established by injecting carrageenan into chest.The rats were sacrificed after 5 h and the blood and the exudates were collected.The ELISA method was used to detect the production of TNF-α,IL-1β,PGE2 in exudates.The NO content was measured by Griess assay.The contents of malondialdehyde(MDA) and the activity of superoxide dismutase(SOD) were detected by kit instruction.Results Compared with acute pleurisy model group,DL0309 decreased the volume of exudates,the total leukocyte numbers and protein contents in exudates.Meanwhile,DL0309 could significantly reduce the levels of IL-1β and PGE2 and decrease the contents of TNF-α and NO.In addition,DL0309 was also able to decrease the contents of MDA and the activity of SOD in plasma in carrageenan-treated rats.Conclusion DL0309 has potent anti-inflammatory effects on carrageenan-induced acute pleurisy model in rats.