The expression of OPG and RANKL in cultured human gingival fibroblasts stimulated with E. coli LPS

Objective:To exam the expression of osteoprotegerin(OPG) and receptor activator of NF-kB ligand(RANKL) in cultured human gingival fibroblasts(HGFs) stimulated with E.coli LPS.Methods:The fourth passage of HGFs were stimulated with 5 μg/ml of LPS for 0,6,12,24 h and 48 h respectively.Immunocytochemistry was used to exam the expression of OPG and RANKL expression,ELISA was used to exam the secretive OPG in the culture supernatant.Results:RANKL expression exhibited no significant change in HGFs after LPS stimulation;while OPG expression increased in a time dependent manner,significant difference was observed for all stimulated groups compared with the control(P0.01).6 h and 12 h stimulation showed significant difference(P0.05).Secretive OPG in HGFs culture supernatant revealed a similar pattern.Conclusion:LPS stimulation can increase OPG expression in HGFs.