Study on the Chemical Components of Volatile Oil from the Leaf of Melia azedrach Linn and its Antimicrobial Activity

[Objective] The technical guidance for the development of Melia azedrach Linn as medicine was provided through the research.[Method] The volatile oil was extracted from Melia azedrach Linn with water-steaming method and the chemical composition of the volatile oil was analyzed with GC-MS.[Results] There were 68 separated peaks after the detection of GC-MS and 39 material were identified,which mainly included alkan(3.75%),esters(3.06%),alcohol30.25%),fatty acid(45.28%),ketone(5.42%) and others.The experiment in the effect of the volatile oil on the anti-microbial activity was conducted and the result indicated that that 25 mg/ml,50 mg/ml,75 mg/ml and 100 mg/ml of the volatile oil could have strong inhibition effect on Escherichia coli,Asperigillus niger and Staphylococcus aureus,which efficiencies were significantly stronger than that of the CK.The order of the inhibition effects of the volatile oil on the three microorganisms was Asperigillus niger Escherichia coli Staphylococcus aureus.[Conclusion] The chemical composition of the volatile oil from Melia azedrach Linn leaf was complex and most of them were with low contain.That was the basis of the inhibition effect on microorganisms.The volatile oil had inhibition effect on microorganism,especially on fungus.