Prokaryotic Expression and Polyclonal Antibody Preparation of SDG711 C-terminal from Rice

[Objective] This study aimed to conduct prokaryotic expression of rice SDG711 C-terminal and prepare its polyclonal antibody.[Method] C-terminal of rice SDG711 containing relatively intensive antigen determinants was selected for prokaryotic expression,prokaryotic expression vector pET28a-711C was constructed and the recombinant plasmid was transformed into Escherichia coli BL21(DE3) competent cells.The recombinant fusion protein was induced by IPTG and purified to immunize a New Zealand white rabbit as the antigen,and polyclonal antibody was obtained for Western-blot analysis.[Result] The polyclonal antibody prepared could efficiently detect the antigen expression.[Conclusion] This study laid the foundation for further investigating the functions of SDG711 protein.