Relationship between pathology in liver tissues and level of serum HBV-DNA,HBeAg of patients with chronic hepatitis B

Objective To study the relationship among pathology in liver tissues and level of hepatitis B virus DNA(HBV-DNA),hepatitis B e antigens(HBeAg) in serum of patients with chronic hepatitis B.Methods The liver biopsy was performed in 55 hospitalized patients.According to hepatitis grades(G) and fibrosis stages(S),the patients were divided into three groups.Hepatic pathology was examined,and the serum HBV-DNA level was tested by amolisensor quantitative polymerase chain reaction and serum HBeAg by enzyme linked immunosorbent assay.Results With the development of liver inflammation from G_(1-2) group to G_3,G_4 group,the serum HBV-DNA decreased((6.19)±(1.36),(5.42)±(1.52),(4.42)±(1.33))(P(0.05)),and HBV-DNA decreased from S_(1-2) group to S_3,S_4 group,too((6.37)±(1.22),(5.51)±(1.52),(4.60)±(1.42))(P(0.05)).The serum HBV-DNA was negative correlated with the hepatitis grades and fibrosis stages(r=(-0.481),(0.501),respectively,P(0.01)).The positive rate of serum HBeAg decreased from G_(1-2) group to G_3,G_4 group((84.2%),(57.1%),(46.7%))(P(0.05));and decreased from S_(1-2) group to S_3,S_4 group,too((87.5%),(61.1%),(47.6%))(P(0.05)).Conclusion With the development of liver fibrosis stages and grades in the patients with chronic hepatitis B,the serum level of HBV-DNA and the positive rate of HBeAg decreased.