Development of monoclonal antibodies against zearalenone

To develop a rapid,simple,sensitive and effective method for detecting zearalenone( ZEN),the hapten ZEN-oxime were synthesized through the reaction between zearalenone and carboxymethoxylamine hemihydrochloride. The hapten was conjugated to carrier protein to form the complete antigens by NHS ester method. One hybridoma cell line( 3D10)secreting monoclonal antibody( McAb) against ZEN was produced by fusing mouse myeloma cells( SP 2 /0) with spleen cells of BALB / C mice immunized by artificial antigen conjugated with bovine serum albumin( BSA). Isotype and subclass of the monoclonal cell line( 3D10) belonged to IgM. The light chain of the McAb was identified as Kappa. The titre of ascitic fluids was up to 1 × l0- 6by indirect ELISA. There were cross reactions between the monoclonal antibodies and ZEN and structural analogues. There was no cross reaction between ZEN monoclonal antibodies and the other three mycotoxins.The IC50of McAb against ZEN was 225 ng / ml. In conclusion,the McAb prepared can be used for preliminary and qualitative detection of ZEN residue.