Studies on protoplast fusion between strain of F46 and strain of SC1, SC11

The key factors affecting the protoplasts formation and fusants regeneration were determined between hyperparasitic Streptomyces F46 and actinomycetes SC1 or/and SCll. The results showed that the suitable concentration of Gly and sucrose for strain F46, SC1 and SCll were 0. 5 and 30 g/mL . The high rate of protoplasts formation and fusants regeneration could be obtained for strain F46 lysing 75 min with macerozyme of 10 mg/mL snailase and 10 mg/mL lysozyme (1:1) in 35℃water bath, and for strains SC1 and SC11 lysing respectively 60 min and 100 min with 10 mg/mL Lysozyme and 15 mg/mL lysozyme in 35℃water bath. The period of thermal inactivation in 55℃was 45 min for strain F46, and was 15 min for stain SC1 and SC11. The suitable inactivation period with UV light was respectively 16, 2 and 3 min for stain F46, SC1 and SC11.