The Growth Suppress Effect of Exogenous p16 Gene on Human Esophageal Carcinoma Cell Lines

Objective To study the effect of transfection exogenous PcDNA3 P16 expression plasmid on proliferation of human esophageal squamous carcinoma cell lines Ec109. Methods According to the different transfected plasmid and whether the transfection were underwent, three group were divided and 5 sample included in each group.P16 transfection group: p16 cDNA was introducted into Ec109 cell line by the vector of Lipofectamine. Empty plasmid transfection: PcDNA3 neo plasmid was introducted with the same method as a blank control group.Non transfection group: the Ec109 cell that were not transfected as a negative control group. Immuno fluorensence microscope and Northern dot hybirdization were used to detect P16 protein status. Methyl tetrazo thialum (MTT) method and flow cytometry were used for analysis of cell cycle and growth inhibition. Cell apoptosis was detected by DNA fragment electrophoresis and flow cytometry. Results The cell vitality of transfected Ec109 cells decreased and the G1 phase arrest detected after PcDNA3 P16 expression plasmid was transfected and expressed in Ec109 cell lines. Cell apoptosis was observed while P16 protein transient expressed, but the other control groups had negative results. Conclusion Transfection exogenous p16 gene to Ec109 conducted by liposome would result in cell growth inhibition. Transient p16 gene expression could induce cell apoptosis.