Analysis on TATA-box,GC-box and CAAT-box in Eukaryotic Promoters

2541 eukaryotic promoter sequences that had been validated by test were downloaded and the number of TATA-box,GC-box and CAAT-box and its distribution conditions in the promoters were analyzed by bioinformatics method.The results showed that 23.85% eukaryotic promoter sequences had at least one TATA-box and TATA-box distributed mainly in the area 23～128 bp ahead of transcription initiation site.47.30% eukaryotic promoter sequences had at least one GC-box and GC-boxes were concentrated in the area 23～128 bp ahead of transcription initiation site.42.35%eukaryotic promoter sequences had at least one CAAT-box and CAAT-boxes were concentrated in the area 51～159 bp ahead of transcription initiation site.The results indicated that the position of TATA-box in eukaryotic promoters was relatively fixed and it might play an important role in the correct initiation of gene transcription.But GC-box and CAAT-box were widely distributed and their number was obviously more than that of TATA-box.