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2004•Marine Science BulletinRequires access

Determination of Chlorophyll a in the Surface Sea Water with HPLC

Shang Longsheng

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Abstract

A HPLC method has been used for determination of chlorophyll-a in the surface sea water of the Liaodong Bay. ODS-2 Spherisorb C18 reversed phase column is used. UV detection wavelenth is 436 nm. The HPLC solvents are methanol, 0.5 mol/L ammonium acetate aaq., acetonitril, water and ethyl acetate. They are all of HPLC-grade. Limit of detection is 10 ng/L. The correlations of three methods (spectrometer, fluorescence, HPLC) are compared.

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What this paper is about

A HPLC method has been used for determination of chlorophyll-a in the surface sea water of the Liaodong Bay. ODS-2 Spherisorb C18 reversed phase column is used. UV detection wavelenth is 436 nm. The HPLC solvents are methanol, 0.5 mol/L ammonium acetate aaq., acetonitril, water and ethyl acetate. They are all of HPLC-grade. Limit of detection is 10 ng/L. The correlations of three methods (spectrometer, fluorescence, HPLC) are compared.

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Available abstract

A HPLC method has been used for determination of chlorophyll-a in the surface sea water of the Liaodong Bay. ODS-2 Spherisorb C18 reversed phase column is used. UV detection wavelenth is 436 nm. The HPLC solvents are methanol, 0.5 mol/L ammonium acetate aaq., acetonitril, water and ethyl acetate. They are all of HPLC-grade. Limit of detection is 10 ng/L. The correlations of three methods (spectrometer, fluorescence, HPLC) are compared.

Key concepts: High-performance liquid chromatography, Ammonium acetate, Chromatography, Ethyl acetate, Detection limit, Chemistry

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