Effects of left ventricular device on c-fos mRNA in myocardial cell of canine with acute left ventricular failure

Objective:To study the effects of domestic pneumatic left ventricular assist pump on c-fos gene expression in myocardial cell of canines with acute left ventricular failure.Methods: 20 healthy dogs were divided at random into three groups: assistant group(group A,n=9),heart failure group(group B,n=9) and control group(group C,n=2).Group A received left ventricular assistance after onset of acute left ventricular failure,group B did not receive left ventricular assistance after onset of acute left ventricular failure and group C was only performed a sterno-operation without acute left ventricular failure.After operation for six hours,the dogs were killed and the levels of C-fos mRNA were detected by RT-PCR.Results: There was no evidence of c-fos mRNA expression in group C.C-fos expression increased markedly in group B and reduced in group A compared with that in group B(P0.01).Conclusion: The domestic pneumatic left ventricular assisst device can inhibit c-fos expression after acute left ventricular failure and protect myocardial cells from secondary injury,which may be one of mechanisms that left ventricular assisst device protect function of left ventricular.