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Convenient preparation of mouse model of retinal neovascularization

Jie Jin

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Abstract

Objective:To establish the mouse model of retinal neovascularization. Methods:Twenty neonatal C57BL/6J mice were divided into two groups randomly.The treated group was exposed to 75% oxygen for 5 days and then to room air.All animals were sacrificed at postnatal day 17 and both eyes were enucleated.Endothelial cell nuclei extending into the internal limiting membrane were counted via HE stain and the expression of vascular endothelial growth factor(VEGF) was detected via immunohistochemical stain. Results:On average,25.70±4.90 endothelial cell nuclei were seen in the treated group and 0.85±1.02 in the control group.The difference was statistically significant(P0.01).Stain of VEGF was weaker in the control group than in the treated group. Conclusion:The mouse model of retinal neovascularization can be established by this convenient method.

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What this paper is about

Objective:To establish the mouse model of retinal neovascularization. Methods:Twenty neonatal C57BL/6J mice were divided into two groups randomly.The treated group was exposed to 75% oxygen for 5 days and then to room air.All animals were sacrificed at postnatal day 17 and both eyes were enucleated.Endothelial cell nuclei extending into the internal limiting membrane were counted via HE stain and the expression of vascular endothelial growth factor(VEGF) was detected via immunohistochemical stain. Results:On average,25.70±4.90 endothelial cell nuclei were seen in the treated group and 0.85±1.02 in the control group.The difference was statistically significant(P0.01).Stain of VEGF was weaker in the control group than in the treated group. Conclusion:The mouse model of retinal neovascularization can be established by this convenient method.

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Available abstract

Objective:To establish the mouse model of retinal neovascularization. Methods:Twenty neonatal C57BL/6J mice were divided into two groups randomly.The treated group was exposed to 75% oxygen for 5 days and then to room air.All animals were sacrificed at postnatal day 17 and both eyes were enucleated.Endothelial cell nuclei extending into the internal limiting membrane were counted via HE stain and the expression of vascular endothelial growth factor(VEGF) was detected via immunohistochemical stain. Results:On average,25.70±4.90 endothelial cell nuclei were seen in the treated group and 0.85±1.02 in the control group.The difference was statistically significant(P0.01).Stain of VEGF was weaker in the control group than in the treated group. Conclusion:The mouse model of retinal neovascularization can be established by this convenient method.

Key concepts: Stain, Neovascularization, Retinal, Immunohistochemistry, Vascular endothelial growth factor, Ophthalmology, Inner limiting membrane, Pathology

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